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HL-1 (小鼠心肌细胞) (种属鉴定正确)
2466 人阅读发布时间：2021-05-31 08:56
| 名称 | HL-1 (小鼠心肌细胞) (种属鉴定正确) |
| 别称 | HL1 |
| 种属 | 小鼠 |
| 年龄（性别） | 雌 |
| 生长特性 | 贴壁细胞 |
| 细胞形态 | 成纤维细胞样 |
| 背景描述 | HL-1 Cardiac Muscle Cell Line is an immortalized mouse cardiomyocyte cell line able to continuously divide and spontaneously contract while maintaining a differentiated cardiac phenotype. HL-1 can be serially passaged without losing its differentiated cardiac myocyte phenotype, including morphological, biochemical, and electrophysiological properties. HL-1 is used in a variety of model systems to address questions of cardiac biology at the cellular & molecular levels. |
| 生物安全等级 | 1 |
| 生长培养基 | MEM(PM150410)＋10% FBS(164210-500)＋1% P/S(PB180120) |
| 推荐传代比例 | 1:4-1:6 |
| 推荐换液频率 | 2~3次/周 |
| 冻存条件 | 冻存液：55% 基础培养基+40%FBS+5%DMSO温度：液氮 |
| 培养条件 | 气相：空气，95%；CO2，5%温度：37℃ |